PyRosetta

Hello :

I am learning the basics of pyrosetta from this tutorial 

https://graylab.jhu.edu/pyrosetta/downloads/documentation/PyRosetta_Manual.pdf

but when I enter the code verbatim I get an error.

is  something wrong with the syntax they give?  trying to do this:

hello everyone,

I was using a script i wrote last year regarding motif grafting, but it no longer works, i suspect a pyrosetta update issue.

anyway, i am looking through the errors and it seemed the pyrosetta.rosetta.protocols.motif_grafting.movers.MotifGraftMover's init_parameters() have some additional arguments that is must have.

my code is as follows:

Hello everyone, 

recently, i doing the Exercises of workshop6, but i found i was confused about the packing and design in rosetta...

Anybody can help to figure out why the two setting method of PACK （def pack1 and def pack2) return different energy？it should be the same as i think！

if i run pack1 followed by pack2, then it return the same(lower) energy. it seens that the design energy is not optimized. so should i set another packing after the design? 

thanks if anyone can help.

MY CODE: 

Hi,

Would anyone know how to create a glycosaminoglycan (heparan sulfate, chondroitin sulfate, hyaluronic acid) using pyrosetta?

Many thanks,

Tarsis