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Inquiry about MPI-Rosetta: Issue with -nstruct Parameter

Category: 
Structure prediction

Subject: Inquiry about MPI-Rosetta: Issue with -nstruct Parameter

Hello Rosetta community,

My name is Serena, and I have a question regarding the MPI-Rosetta forum. I hope to articulate my issue clearly.

Post Situation: 

Broken structure of local docking of replicadock2

Category: 
Docking

Hello, I tested local docking of AlphaRED (ReplicaDock2) protocol. As the mc steps, the fa_rep score increases rapidly.
Checking a trajectory with high fa_rep score, there are clashes between some residues. (e.g. 1st score and 2000th score)

Post Situation: 

Error when running the CstfileToTheozymePDB Application

Category: 
Enzyme Design

Hello,

I am relatively new to Rosetta, and i am trying to generate a constraint file (.cst) with my catalytic amino acid and ligand defined, that i am going to use to search against a PDB database. However, no matter how hard i try i keep obtaining this error:

 

Post Situation: 

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