I'm using antibody protocl to graft some models but it fails with the following error:
ERROR: Unxpected length of light-fr3 [length=31], length expected to be: [32, 33, 34]!
Do you have any comments on this? I have three sequences (three different antibodies with light and heavy chains) and it works on one of them but the same error on the two others.
Thanks a lot in advance,
The framework 3 region in the light chain is an illegal length - 31 instead of 32-34. Rosetta only has templates and tools for sufficiently canonical antibodies. It detects your sequence as too noncanonical for it to work with.