hi
im a doctorial student in the learning process of rosetta scripts
and i was to solve a problem like i got to design a protein complex A0_B0 with known structure and known complex of A1_B1 A2_B2 all Bi are homologs
i need to design a binder A0 that binds to B0
but I just dont know how
and a teacher told me that i needed to manually docking B0 to like a1
and to use fastdesign,fastrelax, to use taskops of layerdesign,limitaromachi2,PruneBuriedUnsats,and filters like BuriedUnsatHbonds
so how am i supossed to know which mover and taskop to use if i am learning by materials and all myself
is that just experience or ?
what if i am to design a denovo binder or to do structure prediction
what combinations of movers and taskops then? ( assume i know all the meaning of single ones)
This task is not easy even for someone with years of Rosetta experience. I would go onto the wiki and start reading. There is documentation for everything you talked about. Go through the referenced papers, tutorials, etc.
thank you for reply
do you mean referenced papers by those who solved a actural problem or those by bakerlab about how some movers or taskops are built?