I want to evaluate the ddg between WT and disulfide bond mutants
1. I try to use ddg_monomer and fond it does not suport it
2. Then I build disulfide bond mutants with moddler, and then try to use ddg_monomer to evaluate the ddg ( mutant CYS recovery to the amino acid of WT), and it does not work , too.
3. Just as 2. I try to use another APP, cartesian_ddg ， and disulfide bond mutants as the star model，and ddgs seem is similar(just try three mutant)
How can I evaluate the ddg between WT and disulfide bond mutants ? Thanks
Could someone please answer my question？