I'm trying to learn enzdes using tutorials in demo and in the paper "de novo enzyme design using rosetta3". The first several steps went well, but when I tried the actual design step, I got this error: ERROR: Illegal value for real option -enzdes:cut1 specified: 6.0. This does not make sense at all. Anyone can help with this? Thanks.
What does your command line and flags file look like?
Here are the flags I used:
Here is the command line:
rosetta3/rosetta_source/bin/enzyme_design.linuxgccrelease -database ~/rosetta3/rosetta_database/ @tim.flags
I don't know why you're getting that error. The best I can come up with is that there is some sort of invisible/non-printing character after the "6.0" which is confusing the options reader. My suggestion would be to re-type the flags file (*don't* copy & paste, lest you copy & paste the non-printing character) and retry it.
Thank you very much for your great help, Rocco! I rewrote the flag file and it works now. It's definitely an error caused by some invisible characters.