Hello, I am looking to use the SEWING protocol to create a new hybrid enzyme from two parent enzymes that are metalloenzymes. I am looking for more information as the documentation pages are slim. Specifically, I wanted to use the new protocol as that takes into account ligand sites, which I figure I'll need to maintain the metal coordination residues. The first stage of creating a motif file. Base on the segment generation page (https://www.rosettacommons.org/docs/latest/scripting_documentation/RosettaScripts/composite_protocols/sewing/Segment-File-Generation) which states you need a motif file. Is there any way to automatically generate this motif file? Or am I going to have to go through each protein structure and determine all the potential motifs that may be present?
I know in the legacy sewing protocol this is automatically done through the features reporting to a database.