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VH-VL Orientation in antibody

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VH-VL Orientation in antibody


How to determine the VH-VL orientation angle in rosetta. i came across this manuscript "Improved prediction of antibody VL–VH orientation" by Protein Eng Des Sel. 2016 Oct; 29(10): 409–418. Here there is description of MT protocol. Whether the script is provided in the rosetta 3.1 version. Or is there any other alternative in rosetta to calculate the VH-VL orientation (LHOC) angle of antibody in rosetta.

Antcipating a reply soon

Thanking you

Dr. Suji george

Scientist C

National Institute of Virology

pune, Maharashtra, INDIA.

Post Situation: 
Mon, 2019-06-17 01:23

Dr. Suji,

I am not 100% certain when the application was moved to be public, but in the newer versions of Rosetta (3.7 and newer), you can run the "packing_angle" application to calculate the four metrics, then the LHOC is just the z-score of those metrics relative to the means/std. devs. described in the paper you are referencing. 

See also for more information on which tools is available when.



Mon, 2019-06-17 06:03