You are here

De novo structure building with medium resolution map

3 posts / 0 new
Last post
De novo structure building with medium resolution map

Dear all,

I am trying to build a model for my protein and I have a medium resolution map (~5-6 A). I tried backbone tracing manually, but many regions are dubious. This protein does not have any homologous protein either, so I am looking for tools to do denovo model building with guided electron density. Is there any available protocol to achieve this?



Post Situation: 
Tue, 2019-07-02 09:07

Hello Buddy,

As with most things in cryo-EM it's hard to say without seeing the map. however you're possibly in the range of our capabilities.

you could try the de-novo protocols we have described at

if that doesn't work, you can email me at or Dr. DiMaio and we could discuss this further.


Tue, 2019-07-02 11:02

Thank you! I will try it!


Tue, 2019-07-02 12:03