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antibody protocol fails

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antibody protocol fails


I'm using antibody protocl to graft some models but it fails with the following error:

File: src/protocols/antibody/grafting/
ERROR: Unxpected length of light-fr3 [length=31], length expected to be: [32, 33, 34]!

Do you have any comments on this? I have three sequences (three different antibodies with light and heavy chains) and it works on one of them but the same error on the two others.

Thanks a lot in advance,


Post Situation: 
Mon, 2019-08-19 07:43

The framework 3 region in the light chain is an illegal length - 31 instead of 32-34.  Rosetta only has templates and tools for sufficiently canonical antibodies.  It detects your sequence as too noncanonical for it to work with.

Wed, 2019-08-21 15:21