I am doing protein-protein interface design using RosettaScripts, and in the .xml file under the demo at:
It has the following line:
<Ddg name="ddG" scorefxn="talaris2014" threshold="-15" repeats="2"/> binding energy calculation; an average of two repeats is computed for better numerical accuracy
Does anybody know what the rationale for that -15 threshold value is? Or for any other value that people use? I'm not sure if it's alright for me to use that value or somehow change it to fit my particular project.