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Multistate design issues with -run:msd_job_dist option and MSDMover

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Multistate design issues with -run:msd_job_dist option and MSDMover

Hi there,

I'm trying to perform multistate design using Rosetta 3.11 to improve antibody binding to five slightly different epitopes. I've been following the tutorial provided by the Meiler Lab and I've been able to successfully prepare my input files, but I'm having issues running the design.

First, when I tried to run the design using the attached .options and .xml files, but I got the following error:

File: src/utility/options/
Option matching -run:msd_job_dist not found in command line top-level context

I then tried replacing the options "-run:msd_job_dist" and "-run:msd_randomize" with "-recon 1" and "-randomize 1" according to the latest list of options ( This seemed to work until I got the error:

File: src/protocols/rosetta_scripts/
Input rosetta scripts XML file "multistate_design_v1.xml" failed to validate against the rosetta scripts schema.

Followed by this further down:

Error messages were:
From line 17:
Error: Element 'MSDMover': This element is not expected.

Please let me know if my decision to replace the two options was correct, and what I can do to resolve the MSDMover error.

Thank you so much in advance for your help!

All the best,

Post Situation: 
Wed, 2020-05-13 18:07

Hi! I got the exact same error. I'm using rosetta3.13, and trying to repeat the multi-state design following antibody MSD tutorial, but always got the MSDMover error as described above.

Looking forward to your help and thanks in advance!


Best regards!

Mon, 2023-03-06 09:50

Hi, have you found the answer ? I think the tutorial about the multistate design is not updated. MSDMover is specific to RECON  algorithm instead of RosettaScripts, so try to use recon.default.linuxgccrelease instead of rosetta_scripts.default.linuxgccrelease.

Hope to help you!

Thu, 2023-08-03 19:47