Dear Rosetta community,
I have been worked using the rosetta scripts over past of few months on protein interface design. The issue is to design the interface of a protein (chain B) into a complex using the PSSM of this protein to drive the mutations. For that, I requested the FavorSequenceProfile before the design (see the script bellow ). A designed sequence was obtained but I am not sure if PSSM was requested during the process, because no task_operations was defined in PackRotamersMover. I tried to use the SeqprofConsenses for this, but it did not work.
All suggestions are welcome!
<ScoreFunction name="ref2015_toPSSM" weights="ref2015">
<Reweight scoretype="res_type_constraint" weight="0.4"/>
<ProteinInterfaceDesign name="pido" interface_distance_cutoff="8.0"/>
<PackRotamersMover name="interface_design" scorefxn="ref2015_toPSSM" task_operations="pido"/>
<FavorSequenceProfile name="FSP" pssm="%%pssm%%" scaling="none" chain="2"/>