# Extending Pyrosetta toturials for docking by adding constraints

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Extending Pyrosetta toturials for docking by adding constraints
#1

Hello,

I followed pyrosetta tutorials for docking.  I did something like the following:

scorefxn_low = create_score_function("interchain_cen")
jd = PyJobDistributor("output", 100, scorefxn_low)
while not jd.job_complete:
cen_pose.assign(starting_cen_pose)
dock_lowres.apply(cen_pose)
jd.output_decoy(cen_pose)

Then, I selected the candiddate with lowest score.

Now, my question is how can I add my own constraints (I have some constraints between CB of residues of chains A and B). I know how to add constraints, but I don't know which part of the above code (or code in toturial for docking) must be changeed in order to include my constraints.

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Thu, 2020-11-26 13:16
nasim.soltani58

You might want ti check out: https://www.rosettacommons.org/node/10951

Wed, 2020-12-02 13:49
matteoferla