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Rosetta remodelling - how to change the added residue type instead of just VAL

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Rosetta remodelling - how to change the added residue type instead of just VAL
#1

Dear sir,

I am new to Rosetta. I followed the rosetta protocol to pad a few residues to N, C terminals of my protein. I follow the remodel.static.linuxgccrelease instructions. Created pad.bd as below. Then I run "rosetta_bin_linux_2021.16.61629_bundle/main/source/bin/remodel.static.linuxgccrelease -in:file:s 1mhp_H_0001.pdb -remodel:blueprint 1mhp_padpadded   -remodel:dr_cycles 1 -num_trajectory 1 -save_top 1" . but it only added Val reisidues. I want to added non-specified residues but not all into VAL. how to fix this? Kind regards.

 

 

97 R .
98 G .
99 F .
100 G .
101 D .
102 G .
103 G .
104 Y .
105 F .
106 D .
107 V .
108 W .
109 G .
110 Q .
111 G .
112 T .
113 L .
114 V .
115 T .
116 V .
117 S .
118 S L
0 x L 
0 x L 
0 x L 
0 x L 
0 x L 
 

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Wed, 2023-02-15 14:00
xuezhi