I'm using floppytail app (rosetta 3.5) to model my protein complex. The first chain in pdb file is a 353 residue protein and the second chain is a 138 residue protein. The "floppytail" region is N-terminal residues 1-37 in the second chain.
In the generated structures I noticed that the N-term residue of second chain (beginning of "floppytail") is not moving in space. All generated structures overlap in first chain(residue 1-353), and the first residue of second chain(residue 354 in pdb). I think the first chain is not supposed to move but I wanted N-term of second chain to move.
I defined flexible region in flag file like this:
And I also tried to add these options, but they did not solve the issue.
Could someone give me some advice?