I need to make same mutation at a single position in all three chains of a homotrimer by using ALLAA (all_natural_aminoacids in the resfile).
I have used the below command to run fixbb for the homotrimer with the symmetry flags, and the fixbb gave output without any error but the output had 30 chains of the monomer(outputfile=16.4MB, inputfile=845KB).
### ~/rosetta-3.4/rosetta_source/bin/fixbb.linuxgccrelease -database ~/rosetta-3.4/rosetta_database/ -s pdb.pdb -resfile resfile.resfile -symmetry:symmetry_definition pdb.symm
I created pdb.symm by running the below command :
### perl ~/rosetta-3.4/rosetta_source/src/apps/public/symmetry/make_symmdef_file.pl -m NCS -p pdb.pdb -a A -i B C > pdb.symm
So how do i change the commands/flags to make proper symmetrical mutations.
Thanks in advance!!!