Dear Rosetta Commons,
I recently wanted to use a set of ambiguous constraints in structure prediction and I prepared a file that looks like this:
AtomPair CB 26 CB 38 BOUNDED 1.5 8.0 1.5 NOE
AtomPair CB 28 CB 61 BOUNDED 1.5 8.0 1.5 NOE
AtomPair CB 26 CB 61 BOUNDED 1.5 8.0 1.5 NOE
Unfortunately, it seems that the ab initio application is ignoring the ambiguous constraints. No constraints are used in the prediction and also there is not constraint score term in the final score file.
Other constraints work just fine.
Anybody encountered this before? Is there a workaround or something wrong in the way I defined the constraints?