# Rosetta membrane blastpgp/psiblast database problem for lips4 generation

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Rosetta membrane blastpgp/psiblast database problem for lips4 generation
#1

I have run Rosetta membrane successful in the past, but it seems that now the blastpgp database necessary to run the run_lips.pl script is obsolete.
In the lastest BLAST release, blastpgp has been dropped entirely in favor of psiblast.
I am then blocked and I cannot generate the lips4 file using:
run_lips.pl <fasta file> <span file> <path to blastpgp> <path to nr database> <path to alignblast.pl script>

Is there a way of the run_lips.pl connecting the psiblast, or does it need to be modified?

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Tue, 2014-09-16 11:45
fbarrera

There is currently an effort to update and modernize the membrane related code in Rosetta, which would include the run_lips script, so updating is something that's on the list of things to do, although we don't have it completed currently.

Here's a quick outline of the changes which would need to be made:

The key line of code in run_lips.pl with respect to blast is line 80, which basically invokes the command

$PSIBLAST -i$seq -d $DB -j 2 -h 0.001 -b5000 -v5000 -o$blastout

To use the more recent version, it looks like that needs to be changed to

$PSIBLAST -query$seq -db DB -num_iterations 2 -evalue 0.001 -num_alignments 5000 -num_descriptions 5000 -oblastout

The output format is also almost-but-not-quite the same, so the alignblast.pl script needs to be changed. I've attached a patch file which shows the necessary changes. (This can be quickly applied with "patch alignblast.pl < alignblast.patch_.txt" - I'd recommend making a backup copy of the old version before you do.)

Note that I haven't benchmarked these changes - There may be some additional setting/parameter changes you would need to make in order to get equivalent performance from the lips predictions for the psiblast versus blastpgp. Caveat experimentor.

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Wed, 2014-09-17 10:06
rmoretti