I'm trying to use FloppyTail application, however it always gives me an error:
[ERROR] EXCN_utility_exit has been thrown from: src/protocols/floppy_tail/FloppyTailMover.cc line: 268
ERROR: invalid flexible region (start (0) or stop (0) is undefined) - check your flags or movemap file
I tried to use it with options from the demo (in /integration/tests), and simply running like
path_to_rosetta/main/source/bin/FloppyTail.default.linuxgccrelease -in:file:s 3UG9.pdb -flexible_start_resnum 236 -flexible_stop_resnum 294
How to define these parameters correctly, cause I couldn't find the answer in --help.
Thank you in advance,
You need to define the chain, too. You will see "-FloppyTail:flexible_chain C" in the options file in the integration test. It wants to interpret 236 and 294 as PDB residue codes, not raw residue indices, so it needs to know which chain they are on.