-in -file #-native 2eu2_A_relax_0001_ideal_0001.pdb -fasta 2EU2.fasta -frag9 frags.200.9mers #-frag9 frags.dsc.500.9mers -frag3 frags.200.3mers #-frag3 frags.dsc.200.3mers -psipred_ss2 2eu2.psipred_ss2 -path -database ~/rosetta_src_2021.16.61629_bundle/main/database/ -auto_setup_metals true -metals_detection_LJ_multiplier 1.0 -metals_distance_constraint_multiplier 1.0 -metals_angle_constraint_multiplier 1.0 -detect_disulf true -detect_disulf_tolerance 1.0 # -constraints_from_link_records true -abrelax -abrelax true -abinitio -abinitio true -relax true #-final_clean_relax true # -multifastrelax # -include_stage5 true -use_filters true # -use_loophash_filter true -increase_cycles 10 -rg_reweight 0.25 -rsd_wt_helix 0.25 -rsd_wt_loop 0.25 -rsd_wt_strand 0.25 # -temperature 1.5 -kill_hairpins 2eu2.psipred_ss2 # -kill_hairpins_frequency 1.0 -no_write_failures true #-relax_failures true -relax_with_jumps true -return_full_atom true -detect_disulfide_before_relax true # -steal_3mers true # -steal_9mers true # -close_loops_by_idealizing true #-MonteCarlo # -temp_initial 4.5 # -temp_final 0.616 -relax -thorough -default_repeats 10 -bb_move true -chi_move true -jump_move true -fix_omega false # -superimpose_to_native true # -constrain_relax_to_native_coords true # -use_explicit_water true -range -kT 0.616 -min_cycles 1000 # -minimize_bond_lengths true # -minimize_bond_angles true #-fold_cst # -no_minimize true -overwrite -run -rebuild_disulf true #-constant_seed #-jran 1234567 #-seed_offset 10 #-nproc 5 -corrections -beta_nov16 true -jumps -residue_pair_jump_file 2eu2.res_pair_jump_cst # -residue_pair_jump_file 2eu21.res_pair_jump_cst # -residue_pair_jump_file 2eu22.res_pair_jump_cst -packing -ex1 # -level 3 -ex2 # -level 3 -ex3 # -level 3 -ex4 # -level 3 -ex1aro # -level 3 -ex2aro # -level 3 -ex1aro_exposed # -level 3 -ex2aro_exposed # -level 3 -no_optH false -optH_MCA true -multi_cool_annealer 10 # -lazy_ig true # -use_input_sc -pH -pH_mode true -keep_input_protonation_state true -value_pH 6.0 -constraints -cst_fa_file HisZn.fa_cst -cst_fa_weight 1.0 -out -nstruct 10 -level 300 -pdb -file # -output_virtual true # -output_virtual_zero_occ true # -silent default.out