-database /apps/codes/rosetta.source.release-334/main/database/
-s ./use.pdb 
-ex1 
-ex2 
-extrachi_cutoff 5 
-ignore_unrecognized_res 
-run:constant_seed 
-nstruct 1000
-jd2:ntrials 10 
-native ./template.pdb
-parser:protocol ./tandem-all20AA_design_ref2015_weight.xml 
-docking:no_filters
-out:path:all ./