DDG filter computes binding score for the complex (threshold=0 only allows complexes with negative binding score) (repeats=3 calculates binding score three times and returns average) (repack=1 repack complex in both bound and unbound states to calculate binding score) SASA filter computes interface solvent-accessible surface area (threshold=800 only allows complexes with greater than 800 Å^2 as per Janin et al., Quarterly Reviews of Biophysics, 2008) Runs local refinement stage of full atom docking perturb the backbone of chain2. Change moves to 1000 for real application Runs full atom side-chain and backbone minimization Iterate through design to find best solution. change trial numbers to higher for real application. Iterate through design to find best solution. change trial numbers to higher for real application. Iterate through design to find best solution. change trial numbers to higher for real application. Runs movers and filters in this order