Rosetta 3.15 Release Highlights

The newest version of Rosetta is now available for download: Download Rosetta 3.15.

In addition to general improvements, Rosetta 3.15 introduces several new protocols focused on modeling and designing small-molecule ligands and noncanonical amino acids. These include:

• REvoLd (Rosetta Evolutionary Ligand) for evolutionary ligand design
• RosettaAMRLD for automated Monte Carlo reaction-based ligand design
• GALigandDock, which integrates cryoEM density to evaluate ligand docking
• New apps and tools such as sim_cryo for cryoEM density simulation, expanded ligand motifs utilities, and a new FakeRotLib approach for NCAAs

Beyond protocols, Rosetta 3.15 adds new movers, filters, and chemistries to expand options for drug design, ligand docking, and structural modeling workflows. Updates to the build system, mmCIF parsing, and quality-of-life improvements across metrics and protocols make the release more robust and compatible with modern toolchains.

For the full list of changes and technical details, see the official Release Notes.