Software Download
The Rosetta Commons community develops and supports a wide range of powerful tools for macromolecular modeling and design. These tools are freely available for non-commercial users, such as those who are a part of non-profits, academic institutions, and government agencies. For commercial users, our open-source tools are also available at no cost. However, access to other tools requires a paid license, which can be obtained through the CoMotion office at the University of Washington.
Expand the sections below for tool-specific documentation, installation, and licensing information. If you have further licensing questions, see our Licensing FAQ page.
Installation Issues, Bug Reports, and Support
For installation problems or bug reports, the best approach is to open an Issue in the GitHub repository after reviewing the installation guide for the tool. This creates a searchable, public record and allows community members to contribute solutions.
For general usage questions or suggestions, we recommend starting a Discussion in the tool’s GitHub repository. This approach broadens visibility, encourages collaboration, and lets maintainers convert your post into an Issue if needed.
If you are a Rosetta Commons member with Slack access, you can also use the tool’s dedicated channel or the #how_do_i_do_x channel to get help and feedback.
Actively Supported Software & Tools
About Rosetta:
Rosetta is software suite for modeling macromolecular structures. Its functionalities include structure prediction, design, and remodeling of proteins and nucleic acids.
License Information:
For non-commercial users (academic, non-profits, government) Rosetta is available for free under a license that can be found here.
A paid license is required for commercial users and can be acquired through the University of Washington's CoMotion office. Licensing revenue supports the Rosetta Commons research community by funding conferences, workshops, summer interns and post-baccalaureate scholars, mini-grants, user support, documentation and code infrastructure and testing.
See our Licensing FAQ for more information.
Documentation:
Download/Installation:
Release Versions (Precompiled Binaries and Source)
Development Version (Build From Source)
Related Links:
Historical (Rosetta Forum):
Have a question about Rosetta or PyRosetta? Check the Rosetta Forum first.
The Rosetta Forum officially closed in mid-2024, but remains available in a read-only format. It still contains helpful answers to many user questions and is an excellent resource for Rosetta developers and users.
The best way to search the forum is by using Google’s site operator:
In Google, put ‘site:forum.rosettacommons.org’ before your search terms.
About PyRosetta:
PyRosetta is a Python-based interface to the Rosetta molecular modeling suite. It enables the user to use Python scripting to build powerful, custom modeling algorithms.
License Information:
For non-commercial users (academic, non-profits, government) PyRosetta is available for free under a license that can be found here.
A paid license is required for commercial users and can be acquired through the University of Washington's CoMotion office. Licensing revenue supports the Rosetta Commons research community by funding conferences, workshops, summer interns and post-baccalaureate scholars, mini-grants, user support, documentation and code infrastructure and testing.
Documentation:
Download/Installation:
Related Links:
Historical (Rosetta Forum):
Have a question about Rosetta or PyRosetta? Check the Rosetta Forum first.
The Rosetta Forum officially closed in mid-2024, but remains available in a read-only format. It still contains helpful answers to many user questions and is an excellent resource for Rosetta developers and users.
The best way to search the forum is by using Google’s site operator:
In Google, put ‘site:forum.rosettacommons.org’ before your search terms.
About RF3:
RF3 (RoseTTAFold3) is part of Foundry and is an all-atom structure prediction tool with implicit chirality representations and atom-level geometric conditioning.
License Information:
Licensed under a BSD3-Clause License. More information can be found here.
Documentation:
Download/Installation:
Related Links:
About MPNN:
MPNN is part of Foundry and is a combination of Ligand, Protein, and Soluble MPNN that allows you to design protein sequences that are ligand-aware or find soluble analogs of membrane proteins.
License Information:
Licensed under a BSD3-Clause License. More information can be found here.
Documentation:
Download/Installation:
Related Links:
About HBDesigner:
HBDesigner is an algorithm that designs highly-connected hydrogen bonding networks that fit the requested design constraints onto an input protein backbone.
License Information:
Licensed under an MIT license. More information can be found here.
Documentation:
Download/Installation:
Some Active Support for Software & Tools
About RFdiffusion3:
RFdiffusion3 is for atom-level protein backbone generation and sequence design.
License Information:
Licensed under a BSD3-Clause License. More information can be found here.
Documentation:
Download/Installation:
Related Links:
About RFdiffusion2:
RFdiffusion2 is for ligand aware backbone generation, specifically created to help with enzyme design.
License Information:
Licensed under a BSD3-Clause License. More information can be found here.
Documentation:
Download/Installation:
About RFdiffusion:
RFdiffusion is an open-source method for backbone structure generation.
License Information:
For non-commercial users (academic, non-profits, government) all RosettaDL tools are available for free under a license that can be found here.
Documentation:
Download/Installation:
Related Links:
About RoseTTAFold:
RoseTTAFold is a deep learning protein folding package.
License Information:
RoseTTAFold is part of the Rosetta-DL bundle of deep learning packages.
For non-commercial users (academic, non-profits, government) all RosettaDL tools are available for free under a license that can be found here.
A paid license is required for commercial users and can be acquired through the University of Washington's CoMotion office. Licensing revenue supports the Rosetta Commons research community by funding conferences, workshops, summer interns and post-baccalaureate scholars, mini-grants, user support, documentation and code infrastructure and testing.
Download/Installation:
Related Links:
About ProteinGenerator:
ProteinGenerator is a generator for sequence-structure pairs using RoseTTAFold.
License Information:
Licensed under an MIT license. More information can be found here.
Download/Installation:
About DeepAb:
DeepAb is a deep learning tool for antibody structure prediction.
License Information:
DeepAb is part of the Rosetta-DL bundle of deep learning packages.
For non-commercial users (academic, non-profits, government) all RosettaDL tools are available for free under a license that can be found here.
A paid license is required for commercial users and can be acquired through the University of Washington's CoMotion office. Licensing revenue supports the Rosetta Commons research community by funding conferences, workshops, summer interns and post-baccalaureate scholars, mini-grants, user support, documentation and code infrastructure and testing.
Download/Installation:
About FvHallucinator:
FvHallucinator is a tool for designing sequences that fold into a desired Fv structure using DeepAb.
License Information:
FvHallucinator is part of the Rosetta-DL bundle of deep learning packages.
For non-commercial users (academic, non-profits, government) all RosettaDL tools are available for free under a license that can be found here.
A paid license is required for commercial users and can be acquired through the University of Washington's CoMotion office. Licensing revenue supports the Rosetta Commons research community by funding conferences, workshops, summer interns and post-baccalaureate scholars, mini-grants, user support, documentation and code infrastructure and testing.
Download/Installation:
About MaSIF:
MaSIF, or Molecular Surface Interaction Fingerprints, is a tool for deciphering patterns in molecular surfaces.
License Information:
MaSIF is part of the Rosetta-DL bundle of deep learning packages.
For non-commercial users (academic, non-profits, government) all RosettaDL tools are available for free under a license that can be found here.
A paid license is required for commercial users and can be acquired through the University of Washington's CoMotion office. Licensing revenue supports the Rosetta Commons research community by funding conferences, workshops, summer interns and post-baccalaureate scholars, mini-grants, user support, documentation and code infrastructure and testing.
Download/Installation:
About Protein-Seq-Des:
Protein-Seq-Des is a tool for protein sequence design from a learned potential.
License Information:
Protein-Seq-Des is part of the Rosetta-DL bundle of deep learning packages.
For non-commercial users (academic, non-profits, government) all RosettaDL tools are available for free under a license that can be found here.
A paid license is required for commercial users and can be acquired through the University of Washington's CoMotion office. Licensing revenue supports the Rosetta Commons research community by funding conferences, workshops, summer interns and post-baccalaureate scholars, mini-grants, user support, documentation and code infrastructure and testing.
Download/Installation:
About AF2 Peptide Hallucination:
AF2 Peptide Hallucination is a generator for binders to flexible peptides using AlphaFold2.
License Information:
Licensed under a BSD License. More information can be found here.
Download/Installation:
About RFantibody:
RFantibody is a tool for structure-based de novo antibody design.
License Information:
Licensed under an MIT license. More information can be found here.
Download/Installation:
Historical Software & Tools (No Active Support)
About trRosetta2:
trRosetta2 is a repository for publicly available deep learning models developed in the Rosetta community.
License Information:
trRosetta2 is part of the Rosetta-DL bundle of deep learning packages.
For non-commercial users (academic, non-profits, government) all RosettaDL tools are available for free under a license that can be found here.
A paid license is required for commercial users and can be acquired through the University of Washington's CoMotion office. Licensing revenue supports the Rosetta Commons research community by funding conferences, workshops, summer interns and post-baccalaureate scholars, mini-grants, user support, documentation and code infrastructure and testing.
Download/Installation:
About Privileged Residues:
Privileged Residues are methods for placing residues on the surface of a target protein that can be added to RIFDock.
License Information:
Licensed under an Apache software license. More information can be found here.
Documentation:
Privileged Residues Documentation
Download/Installation:
About BCL with Rosetta Integration:
BCL with Rosetta Integration is an open-source package containing tools for biological and chemical research.
License Information:
Licensed under an MIT license. More information can be found here.
Download/Installation:
About Fork for AlphaFold:
Fork for AlphaFold is an implementation of the inference pipeline of AlphaFold v2.0 and AlphaFold-Multimer.
License Information:
Licensed under an Apache software license. More information can be found here.
Download/Installation:
About Fork for AutoRotLib:
Fork for AutoRotLib is a tool for parameterizing non-cannonical amino acids.
License Information:
OpenEye license required. Learn more here.
Download/Installation: