I want to predict the effect of a point mutation in a protein stability. I have the .pdb crystal structure of the wild type.
After a literature search, I think this paper presents the most comprehensive study of different methods for this analysis DOI:10.1002/prot.22921. So I want to do the prediction using their protocol 16. I also found python code for this protocol here: https://github.com/Kortemme-Lab/ddg/tree/master/protocols/ddg_monomer_16.
My question is if anyone has setup a Rosetta Scripts .xml file to reproduce this protocol?