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proteins.pdb: Not enough chains in submitted protein! [At least 2 chains should be present, found chains: [u'A']...]

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proteins.pdb: Not enough chains in submitted protein! [At least 2 chains should be present, found chains: [u'A']...]
#1

Hello,

 

I am trying to dock two proteins but I keep getting this error. I am not sure why its there as there are two chains in the pdb.

Can anyone help?

Thank you

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dockin_test_rosseta.pdb415.69 KB
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Mon, 2018-03-19 13:34
Loki01

It looks like you're separating the two chains with an ENDMDL tag. By default Rosetta will only read the first model of a multi-model file, so you're only getting the first chain.

Change the ENDMDL lines to TER lines, and you should be good.

Mon, 2018-03-19 13:40
rmoretti

Fantastic, thank you!

Mon, 2018-03-19 13:45
Loki01