According to the Rosetta density tutorial:
"Generally, we select the best 20% of models by geometry, and selecting the best overall by free FSC. The top 5 models should be inspected for model convergence as well as visually inspected for density map agreement"
I would appreciate if someone explains why we choose the lowest FSC score, and what does it mean to have a negatrive FSC score? Also, according to the paper quoted below, shouldn't we sample for the highest free FSC and bnot the lowest?
"this integrated FSC on an independent map (or free FSC) correlates with model accuracy quite well, particularly at high resolution. Furthermore, the real space
correlation between models and the independent testing map over segments of the chain correlates with the local accuracies of models. In high-resolution maps, as the local correlation decreases, the fraction of incorrectly modeled residues increases".