I would like to redesign the dimeric interface using the heterodimer structure of protein A and protein B. I searched several protocols and saw how to increase affinity or stability by fixing one of them and changing the other. However, I want to increase the binding affinity between the two proteins by changing both sides at the dimeric interface. Anyone know how to do that? Has this method been tried before? If there are papers or protocols that I can refer to, please let me know.
Any help will be very appreciated.