I was trying to study the effect of post-translational modifications on the structure of proteins. For that purpose, I followed two different approaches:
I modified the residue with a variant using
pyrosetta.rosetta.core.pose.add_variant_type_to_residue. Then, I replaced the original residue with the modified one using
I tried using
pyrosetta.rosetta.core.pose.add_variant_type_to_pose_residueto directly modify the residue in the pose.
In both cases, I obtained None as a result, but I would like to obtain a modified pose in order to calculate the ΔΔG value. Does anyone know how I can achieve that? Any suggestions would be appreciated.
Thanks in advance,