What's the sign of ddG score in rosetta 3?
I think the ddG here is dG(mut)-dG(wild). so a lower score of mutant means better structure. So negative ddG means stabilize?
But in the paper "Role of conformational sampling in computing mutation-induced changes in protein structure and stability". It looks like ddG from rosetta has same sign as protherm database, which negative sign means destabilize.
So I am confused. Can anyone explain a little bit? Thank you!