I submitted one job for anitbody variable domain structure prediction through Rosie recently. Rosie generated the pdb files. However, when I checked it through Chimera, we found the light chian C-terminal lost two residues (-RA). I double checked my submiting sequence, it was fine. Does anybody meet the similar issue? How could that happen and how to deal with it?
Hi. I got someone on the antibody team to respond. This is a bug and will be marked as such on the bugtracker.
"It probably has to do with the light chain framework that we picked. Those residues must just be missing in it."