Designing Feasible Backbones
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Hello,
I am designing a macrocycle with simple_cycpep_predict that contains a D-Leu in the N-terminal and an acetylated ASP in the C-terminal. My sequence_file (-cyclic_peptide:sequence_file) is DLE ........ ASP
1) When I run the app it doesn't recognize the D-Leu name:
Hi everyone,
I am trying to design a nanobody using an available antibody. After grafting heavy chains from an antibody to a known nanobody scaffold, and aligning with the antibody-antigen complex structure, I obtain nanobody-antigen compelx structure. I am trying to use Rosetta design to increase its affinity to antigen as well as sample possible new CDR sequences using the options,
Hi all,
I performed antibody-antigen docking experiments following the Rosetta antibody-antigen docking protocols. For each antibody-antigen pair, I compared the interface scores (I_sc) among the different antigens with small difference in sequence.
Hello,
We're trying to incorporate a nonanonical amino acid (3, 5-Difluorotyrosine) in my enzyme design protocol. We've been using the molfile_to_params_polymer.py script provided in the demos folder; however, the params file isn't generating correctly (the file is attached below as DFT.params). When running enzyme design protocol, we get the following error:
"Only the third ICOOR atom in a topology file should list itself as its own dihedral atom"
Dear all,
I'm really struggeling to repeat the published procedures to run ReplicaDock2.0 (Local and Global), let alone adopt it to my needs.
Hello,
I am getting the following error:
ERROR: Unable to open weights/patch file. None of (./)ref2015 or (./)ref2015.wts or /cluster/rosetta/bin/mpi/../database/scoring/weights/ref2015 or /cluster/rosetta/bin/mpi/../database/scoring/weights/ref2015.wts exist
Hi there,
I tried to do antibody modeling, however, there is complie issue shown as the following:
$ROSETTA/bin/antibody.linuxgccrelease -fasta 27666.fasta | tee grafting.log
Hi,
I was wondering if it is posible to measure the free energy or stability of one protein in diferent temperature environments. Would this be done by changing the temperature of the energy minimization? And if so, how do I do it? I haven't found a specific flag for this purpose.
Thanks.
"Submitting Rosetta-Vienna RNP-ddG job failed!
Validation failed as:
Please correct this errors and re-submit!"