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Dear community, 

I am just beginning to explore Rosetta with some mutational analysis. 

Currently exploring ddg_monomer. 

From what I have read so far, ddg_monomer doesn't have parallel support. 

Could I please know if setting 

-ddg::iterations 1

and then repeating the above 49 other times, to make a total of 50 predictions and then choosing the top three stated in the manual would produce similar results as setting 

-ddg::iterations 50

and running only once. 

I encountered this error while trying to login to ROSIE:Your GitHub account does not have verified academic email address!

My academic email ( YXIE11@bwh.harvard.edu) cannot be verified during login to rosie. 

Hi all,

I want to generate cyclic peptides in a binding site employing the anchor design approach similar to https://www.nature.com/articles/s41467-021-23609-8 and  https://www.pnas.org/doi/suppl/10.1073/pnas.2012800118. I need to do the closure by amidation between two side chains (K/E) or side chain/C-terminal.

I was trying to use Rosettascript to perform a grafting task. I found that an intermediate pose of my task has the following discontinuity of residue number.