# Help with ddg_monomer in pyrosetta

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Help with ddg_monomer in pyrosetta
#1

I am new to pyrosetta - how can I implement ddg_monomer in pyrosetta ?

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Wed, 2018-01-10 08:30
The ddg_monomer application is a little tricky, as there's a fair amount of setup/analysis it does which isn't encapsulated by a PyRosetta-accessible Mover, though the main part of the calculation is done from within the rosetta.protocols.ddg.ddGMover mover. (Be careful: there's a number of ddG movers in Rosetta - for the ddg_monomer application, it's the one in rosetta.protocols.ddg that you want.)
One of note it setting the wildtype comstuffparison. To do this, create another ddGMover object (with all aa_unks),  run it on the wildtype structure, and then take the result of wt_score_components() (wth no arguments) from the wildtype run and pass it to the wt_score_components() method of the ddGMover you're using to do the mutations.