I am an undergraduate student researching with Pyrosetta for my lab this summer, and I am having difficulties appending/inserting residues into a pose. I would love some help.
I have tried using the grafting protocols (insert_pose_into_pose) but I just realized that there are methods such as append_polymer_residue_after_seqpos which seem to do what I want, perhaps more accurately.
My question is, essentially, if I have a pose with sequence "ABABA" and I want to insert an amino acid "C" after the third residue, how do I do this using the available pose methods?
I thank you greatly for any help you can offer