Hi Rosetta community,
After finally getting the RASREC run to successfully generate full 6 stages, now I'm at the point where I want to score the pdbs to find the lowest energy structures which suppose to be the closest to the native structure. But in this website: https://www.rosettacommons.org/demos/latest/protocol_capture/rasrec_evolutionary_restraints/README
there're no instructions on plotting, however, there's a script for finding 30 structures with the lowest scoring value from the silent.out file.
The script is this:
extract_decoys decoys.out -score 30 > low_30.out
This generates 30 structures with the lowest energy value, which then I converted to pdbs. Now I'm wondering, can I use a similar script to produce one structure with the lowest energy? I used this script to generated one structure with the lowest energy value "extract_decoys low_30.out -score 1 > low_1.out".
The structure I got seems legit, meaning that it seems to incoporate all the restraints I added to the run (Chemical Shifts, membrane restraints). Do you think it is suffeicnet to just leave it at that or am i missing something?
I would appriciate your advice.
I don't have much experience with RASREC, but I talked with someone who should know, and he suggested you post to http://csrosetta.chemistry.ucsc.edu/forum on the CS-Rosetta website, to see if people there can help.
Thanks rmoretti! But the link to the forum you gave me doesn't allow me to post anything. Last post in it is 9 months ago.
RASREC has poor documentations and it's not helpful at all having an inactive forum to top that!