Scoring

Hi,

I am trying to load a structure from PDB and then calculate pairwise residue energies between residues in the structure. For this, I want to first load the scorefxn and then the structure (or viceversa), but whenever I try, either of them gives me a segfault, i.e. if I do scorefxn = get_fa_scorefxn(); pose_from_pdb() the latter crashes and if I do the opposite, get_fa_scorefxn() crashes. 

I am trying to build glycans into a cryoEM density following the Meiler Lab tutorial here: http://www.meilerlab.org/jobs/downloadfile/name/Apr2019_glycan_modeling_talk.pdf

Using the provided XML file, I am able to run the job, but I see the following warning throughout the output file:

0mcore.scoring.electron_density.FastDensEnergy: ^[[0m^[[1m[ WARNING ]^[[0m Fold tree is not set properly for density scoring!

Hi all,

I've recently been asked to try to use RosettaScripts for some enzyme design. In this particular case I am trying to use the HBNet mover to predict hydrogen bond network mutations. I have created scripts with just the HBNet mover and also calling the Multiple Pose mover afterwards. However, I am having the same issue. I don't know how to score the resulting binary silent file.

I am trying to select the top 5 results and extract their PDBs.

Some common errors include:

Hello all, 

            Is it possible to run InterfaceAnalyzer with a structure with Glycans? Everytime I apply the InterfaceAnalyzerMover on my structure I get a segmentation fault. If I just delete the carbohydrates it works just fine. The pose has been processed with all of the usual flags(below). Is there some trick to using glycans and InterfaceAnalyzerMover or can someone point me in the right direction? Thanks so much.

                                                                                                        Drew