Unsolved

Hi,

I have a protein with a loop and I want to made a single mutation or small insertion in the loop.
Do I need to prepare a new fragment file for protein which has a single mutation in the loop, or can I use the fragment file from parent protein?
Is it possible to avoid regeneration of the fragment file, as it takes a lot of time?

Thank you for you help.

Victor

hello, is there any possibility of getting a pyrosetta source distribution that can be compiled with a non system-supplied python (i.e. from macports) on mac osx (10.5+)? i would greatly appreciate this as i am trying to incorporate pyrosetta into a GUI that uses external modules such as OpenGL, PyQt, PyMOL etc. and to me it would seem much easier to compile pyrosetta with a macports version of python than using the system python and having to go through the seemingly painful process of installing all the other dependencies. but i might be wrong.

thanks,
merc

The PyMOL_Mover is working but PyMOL_Observer() complains. Hope you can help:

MacOS 10.7.3, 64 bit system

Python 2.7.1 (r271:86832, Jul 31 2011, 19:30:53)
Type "copyright", "credits" or "license" for more information.

IPython 0.12 -- An enhanced Interactive Python.
? -> Introduction and overview of IPython's features.
%quickref -> Quick reference.
help -> Python's own help system.
object? -> Details about 'object', use 'object??' for extra details.

In [1]: from rosetta import *
init()
In [2]: init()

HI,

I am interested in mutating some loop residues together with the residues of the beta-strand , the loop is connected with. In such case what has to be done?.. Does fixedbb design will work in this case ??

Also , I have another query regarding Rosetta Scoring. I want to test all the 20 possibility of amino acids at two loop residues (20*20=400) of a beta hairpin . In that case will there be any large change in energies of a pair of combination of amino acids ??